human mouse sonic hedgehog shh n terminus antibody Search Results


human mouse sonic hedgehog shh n terminus antibody  (R&D Systems)
93
R&D Systems human mouse sonic hedgehog shh n terminus antibody
Human Mouse Sonic Hedgehog Shh N Terminus Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+mouse+sonic+hedgehog+shh+n+terminus+antibody/bio_rxiv__64898__2026__02__23__707386-25-7-17?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
human mouse sonic hedgehog shh n terminus antibody - by Bioz Stars, 2026-07
93/100 stars
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shh nter  (R&D Systems)
96
R&D Systems shh nter
Expression of <t>Shh</t> mRNA and <t>SHH-Nter</t> protein in the developing cortex. Expression of Shh transcripts detected by in situ hybridization and RNAscope in median (A) and caudal (B) E14.5 brain sections. In situ hybridization of forebrain sections with an antisens Shh probes shows strong expression of Shh mRNA in the ventral forebrain [MGE and MGE/LGE/septum boundary (A1), CGE (B1)], in the zona limitans (ZLI) of dorsal thalamus and the border of the 3 rd ventricle (B1). RNAscop of forebrain sections further confirmed the strong expression of Shh mRNA in the MGE/septum boundary that express Lhx-6 mRNA (a transcription factor expressed in post-mitotic interneurons in the ventral brain) (A2) and the CGE (B2). At higher magnification, Shh mRNA fluorescence (in green) labels a subpopulation of interneuron cell bodies (in red) in the MGE/septum boundary, in the subventricular zone of the MGE and in more dispersed cells in the MGE mantle (A2) and in the lateral and dorsal cortex (arrows, C1 and C2). Among Shh mRNA expressing cells in the cortex, some are principal glutamatergic cells (arrowhead, C1) and only few cIN express the transcript (C3). The graph represents the mean ± SEM in the IZ-CP layers and MZ layer. Expression of SHH-Nter protein in E14.5 median brain sections (D). Representative images are captured with epifluorecence (D1) and confocal microscop (D2). Confocal images are a merged stacks of 10 images and enlargment of pictures are shown. SHH expression is detected in blood vessels and in brigth dots all over the cortical neuropile. At the PSB and in the lateral part of the LGE, SHH-Nter is detectable in brigth elements aligned along the ventricular/subventricular axis. CX, cortex ; LGE, MGE and CGE, lateral, medial and caudal ganglionic eminence, V3, third ventricle; ZLI, zona intra-thalamica; Th, thalamus; Hyp, hypothalamus; PSB, palium/subpallium boundary; VZ, ventricular zone; SVZ, subventricular zone; IZ, intermediate zone; CP, cortical plate; MZ, marginal zone. Scale bar: 500 µm (A, B, D1), 20 µm (C), 50 µm (D2).
Shh Nter, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+mouse+sonic+hedgehog+shh+n+terminus+antibody/bio_rxiv__2024__03__21__586159-210-5-7?v=R%26D+Systems
Average 96 stars, based on 1 article reviews
shh nter - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
human mouse sonic hedgehog shh n terminus pe conjugated antibody  (R&D Systems)
N/A
The Human Mouse Sonic Hedgehog Shh N Terminus PE conjugated Antibody from R D Systems is a rat monoclonal antibody to Sonic Hedgehog Shh This antibody reacts with human mouse The Human Mouse Sonic Hedgehog
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Expression of Shh mRNA and SHH-Nter protein in the developing cortex. Expression of Shh transcripts detected by in situ hybridization and RNAscope in median (A) and caudal (B) E14.5 brain sections. In situ hybridization of forebrain sections with an antisens Shh probes shows strong expression of Shh mRNA in the ventral forebrain [MGE and MGE/LGE/septum boundary (A1), CGE (B1)], in the zona limitans (ZLI) of dorsal thalamus and the border of the 3 rd ventricle (B1). RNAscop of forebrain sections further confirmed the strong expression of Shh mRNA in the MGE/septum boundary that express Lhx-6 mRNA (a transcription factor expressed in post-mitotic interneurons in the ventral brain) (A2) and the CGE (B2). At higher magnification, Shh mRNA fluorescence (in green) labels a subpopulation of interneuron cell bodies (in red) in the MGE/septum boundary, in the subventricular zone of the MGE and in more dispersed cells in the MGE mantle (A2) and in the lateral and dorsal cortex (arrows, C1 and C2). Among Shh mRNA expressing cells in the cortex, some are principal glutamatergic cells (arrowhead, C1) and only few cIN express the transcript (C3). The graph represents the mean ± SEM in the IZ-CP layers and MZ layer. Expression of SHH-Nter protein in E14.5 median brain sections (D). Representative images are captured with epifluorecence (D1) and confocal microscop (D2). Confocal images are a merged stacks of 10 images and enlargment of pictures are shown. SHH expression is detected in blood vessels and in brigth dots all over the cortical neuropile. At the PSB and in the lateral part of the LGE, SHH-Nter is detectable in brigth elements aligned along the ventricular/subventricular axis. CX, cortex ; LGE, MGE and CGE, lateral, medial and caudal ganglionic eminence, V3, third ventricle; ZLI, zona intra-thalamica; Th, thalamus; Hyp, hypothalamus; PSB, palium/subpallium boundary; VZ, ventricular zone; SVZ, subventricular zone; IZ, intermediate zone; CP, cortical plate; MZ, marginal zone. Scale bar: 500 µm (A, B, D1), 20 µm (C), 50 µm (D2).

Journal: bioRxiv

Article Title: KIF7 deletion in a mouse model of human ciliopathy alters cerebral cortex development

doi: 10.1101/2024.03.21.586159

Figure Lengend Snippet: Expression of Shh mRNA and SHH-Nter protein in the developing cortex. Expression of Shh transcripts detected by in situ hybridization and RNAscope in median (A) and caudal (B) E14.5 brain sections. In situ hybridization of forebrain sections with an antisens Shh probes shows strong expression of Shh mRNA in the ventral forebrain [MGE and MGE/LGE/septum boundary (A1), CGE (B1)], in the zona limitans (ZLI) of dorsal thalamus and the border of the 3 rd ventricle (B1). RNAscop of forebrain sections further confirmed the strong expression of Shh mRNA in the MGE/septum boundary that express Lhx-6 mRNA (a transcription factor expressed in post-mitotic interneurons in the ventral brain) (A2) and the CGE (B2). At higher magnification, Shh mRNA fluorescence (in green) labels a subpopulation of interneuron cell bodies (in red) in the MGE/septum boundary, in the subventricular zone of the MGE and in more dispersed cells in the MGE mantle (A2) and in the lateral and dorsal cortex (arrows, C1 and C2). Among Shh mRNA expressing cells in the cortex, some are principal glutamatergic cells (arrowhead, C1) and only few cIN express the transcript (C3). The graph represents the mean ± SEM in the IZ-CP layers and MZ layer. Expression of SHH-Nter protein in E14.5 median brain sections (D). Representative images are captured with epifluorecence (D1) and confocal microscop (D2). Confocal images are a merged stacks of 10 images and enlargment of pictures are shown. SHH expression is detected in blood vessels and in brigth dots all over the cortical neuropile. At the PSB and in the lateral part of the LGE, SHH-Nter is detectable in brigth elements aligned along the ventricular/subventricular axis. CX, cortex ; LGE, MGE and CGE, lateral, medial and caudal ganglionic eminence, V3, third ventricle; ZLI, zona intra-thalamica; Th, thalamus; Hyp, hypothalamus; PSB, palium/subpallium boundary; VZ, ventricular zone; SVZ, subventricular zone; IZ, intermediate zone; CP, cortical plate; MZ, marginal zone. Scale bar: 500 µm (A, B, D1), 20 µm (C), 50 µm (D2).

Article Snippet: Primary antibodies were goat anti SHH-Nter (1:100, R&D system AF464), goat anti Netrin G1a (NG1a) (1:100, R&D system AF1166), rabbit anti TBR1 (1:1000, Abcam ab31940), rabbit anti TBR2 (1:1000, Abcam ab23345), rabbit anti PAX6 (1:100, clone poly19013, Covance PRB-278P), rabbit anti GSH2 (1:2000, Millipore ABN162), chicken anti MAP2 (1:500, Novus, NB30213), and rat CTIP2 (1:1000, Abcam ab18465).

Techniques: Expressing, In Situ Hybridization, RNAscope, Fluorescence